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e coli atcc 11775 recipient strains  (ATCC)


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    ATCC e coli atcc 11775 recipient strains
    E Coli Atcc 11775 Recipient Strains, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 2649 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e+coli+atcc+11775+recipient+strains/Escherichia+coli+(Migula)+Castellani+and+Chalmers/pm40424468-332-12-14
    Average 99 stars, based on 2649 article reviews
    e coli atcc 11775 recipient strains - by Bioz Stars, 2026-10
    99/100 stars

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    Mutagenesis:

    Article Title: An interbacterial cysteine protease toxin inhibits cell growth by targeting type II DNA topoisomerases GyrB and ParE
    Article Snippet: To generate mutations of E. coli ATCC-11775 strains, mutant constructs in pRE118 plasmid were transformed into E. coli S17-1 λ pir. .. E. coli S17-1 λ pir donor strains carrying the mutant constructs and E. coli ATCC-11775 recipient strains to be mutated were grown overnight on LB plates containing appropriate antibiotics, then scraped together to create a 1:1 mixture of each donor-recipient pair that was spread on an LB agar plate and incubated at 37 °C for 6 h to facilitate plasmid transfer via conjugation. .. Cell mixtures were then scraped into LB and plated on LB agar containing kanamycin and chloramphenicol to select for E. coli ATCC-11775 with integrated plasmid.

    Article Title: An interbacterial cysteine protease toxin inhibits cell growth by targeting type II DNA topoisomerases GyrB and ParE.
    Article Snippet: Insertions at the Tn7 sites of E. coli strains were generated using the pUC18mini R6K vector with the target genes amplified by PCR and inserted into the vector through Gibson assembly at the XhoI and SacI restriction sites [100]. .. E. coli S17-1 λ pir donor strains carrying the mutant constructs and E. coli ATCC-11775 recipient strains to be mutated were grown overnight on LB plates containing appropriate antibiotics, then scraped together to create a 1:1 mixture of each donor-recipient pair that was spread on an LB agar plate and incubated at 37 °C for 6 h to facilitate plasmid transfer via conjugation. .. Cell mixtures were then scraped into LB and plated on LB agar containing kanamycin and chloramphenicol to select for E. coli ATCC-11775 with integrated plasmid.

    Article Title: An Interbacterial Cysteine Protease Toxin Inhibits Cell Growth by Targeting Type II DNA Topoisomerases GyrB and ParE
    Article Snippet: To generate mutations of E. coli ATCC-11775 strains, mutant constructs in pRE118 plasmid were transformed into E. coli S17-1 λ pir. .. E. coli S17-1 λ pir donor strains carrying the mutant constructs and E. coli ATCC-11775 recipient strains to be mutated were grown overnight on LB plates containing appropriate antibiotics, then scraped together to create a 1:1 mixture of each donor-recipient pair that was spread on an LB agar plate and incubated at 37°C for 6 hours to facilitate plasmid transfer via conjugation. .. Cell mixtures were then scraped into LB and plated on LB agar containing kanamycin and chloramphenicol to select for E. coli ATCC-11775 with integrated plasmid.

    Construct:

    Article Title: An interbacterial cysteine protease toxin inhibits cell growth by targeting type II DNA topoisomerases GyrB and ParE
    Article Snippet: To generate mutations of E. coli ATCC-11775 strains, mutant constructs in pRE118 plasmid were transformed into E. coli S17-1 λ pir. .. E. coli S17-1 λ pir donor strains carrying the mutant constructs and E. coli ATCC-11775 recipient strains to be mutated were grown overnight on LB plates containing appropriate antibiotics, then scraped together to create a 1:1 mixture of each donor-recipient pair that was spread on an LB agar plate and incubated at 37 °C for 6 h to facilitate plasmid transfer via conjugation. .. Cell mixtures were then scraped into LB and plated on LB agar containing kanamycin and chloramphenicol to select for E. coli ATCC-11775 with integrated plasmid.

    Article Title: An interbacterial cysteine protease toxin inhibits cell growth by targeting type II DNA topoisomerases GyrB and ParE.
    Article Snippet: Insertions at the Tn7 sites of E. coli strains were generated using the pUC18mini R6K vector with the target genes amplified by PCR and inserted into the vector through Gibson assembly at the XhoI and SacI restriction sites [100]. .. E. coli S17-1 λ pir donor strains carrying the mutant constructs and E. coli ATCC-11775 recipient strains to be mutated were grown overnight on LB plates containing appropriate antibiotics, then scraped together to create a 1:1 mixture of each donor-recipient pair that was spread on an LB agar plate and incubated at 37 °C for 6 h to facilitate plasmid transfer via conjugation. .. Cell mixtures were then scraped into LB and plated on LB agar containing kanamycin and chloramphenicol to select for E. coli ATCC-11775 with integrated plasmid.

    Article Title: An Interbacterial Cysteine Protease Toxin Inhibits Cell Growth by Targeting Type II DNA Topoisomerases GyrB and ParE
    Article Snippet: To generate mutations of E. coli ATCC-11775 strains, mutant constructs in pRE118 plasmid were transformed into E. coli S17-1 λ pir. .. E. coli S17-1 λ pir donor strains carrying the mutant constructs and E. coli ATCC-11775 recipient strains to be mutated were grown overnight on LB plates containing appropriate antibiotics, then scraped together to create a 1:1 mixture of each donor-recipient pair that was spread on an LB agar plate and incubated at 37°C for 6 hours to facilitate plasmid transfer via conjugation. .. Cell mixtures were then scraped into LB and plated on LB agar containing kanamycin and chloramphenicol to select for E. coli ATCC-11775 with integrated plasmid.

    Incubation:

    Article Title: An interbacterial cysteine protease toxin inhibits cell growth by targeting type II DNA topoisomerases GyrB and ParE
    Article Snippet: To generate mutations of E. coli ATCC-11775 strains, mutant constructs in pRE118 plasmid were transformed into E. coli S17-1 λ pir. .. E. coli S17-1 λ pir donor strains carrying the mutant constructs and E. coli ATCC-11775 recipient strains to be mutated were grown overnight on LB plates containing appropriate antibiotics, then scraped together to create a 1:1 mixture of each donor-recipient pair that was spread on an LB agar plate and incubated at 37 °C for 6 h to facilitate plasmid transfer via conjugation. .. Cell mixtures were then scraped into LB and plated on LB agar containing kanamycin and chloramphenicol to select for E. coli ATCC-11775 with integrated plasmid.

    Article Title: An interbacterial cysteine protease toxin inhibits cell growth by targeting type II DNA topoisomerases GyrB and ParE.
    Article Snippet: Insertions at the Tn7 sites of E. coli strains were generated using the pUC18mini R6K vector with the target genes amplified by PCR and inserted into the vector through Gibson assembly at the XhoI and SacI restriction sites [100]. .. E. coli S17-1 λ pir donor strains carrying the mutant constructs and E. coli ATCC-11775 recipient strains to be mutated were grown overnight on LB plates containing appropriate antibiotics, then scraped together to create a 1:1 mixture of each donor-recipient pair that was spread on an LB agar plate and incubated at 37 °C for 6 h to facilitate plasmid transfer via conjugation. .. Cell mixtures were then scraped into LB and plated on LB agar containing kanamycin and chloramphenicol to select for E. coli ATCC-11775 with integrated plasmid.

    Article Title: An Interbacterial Cysteine Protease Toxin Inhibits Cell Growth by Targeting Type II DNA Topoisomerases GyrB and ParE
    Article Snippet: To generate mutations of E. coli ATCC-11775 strains, mutant constructs in pRE118 plasmid were transformed into E. coli S17-1 λ pir. .. E. coli S17-1 λ pir donor strains carrying the mutant constructs and E. coli ATCC-11775 recipient strains to be mutated were grown overnight on LB plates containing appropriate antibiotics, then scraped together to create a 1:1 mixture of each donor-recipient pair that was spread on an LB agar plate and incubated at 37°C for 6 hours to facilitate plasmid transfer via conjugation. .. Cell mixtures were then scraped into LB and plated on LB agar containing kanamycin and chloramphenicol to select for E. coli ATCC-11775 with integrated plasmid.

    Plasmid Preparation:

    Article Title: An interbacterial cysteine protease toxin inhibits cell growth by targeting type II DNA topoisomerases GyrB and ParE
    Article Snippet: To generate mutations of E. coli ATCC-11775 strains, mutant constructs in pRE118 plasmid were transformed into E. coli S17-1 λ pir. .. E. coli S17-1 λ pir donor strains carrying the mutant constructs and E. coli ATCC-11775 recipient strains to be mutated were grown overnight on LB plates containing appropriate antibiotics, then scraped together to create a 1:1 mixture of each donor-recipient pair that was spread on an LB agar plate and incubated at 37 °C for 6 h to facilitate plasmid transfer via conjugation. .. Cell mixtures were then scraped into LB and plated on LB agar containing kanamycin and chloramphenicol to select for E. coli ATCC-11775 with integrated plasmid.

    Article Title: An interbacterial cysteine protease toxin inhibits cell growth by targeting type II DNA topoisomerases GyrB and ParE.
    Article Snippet: Insertions at the Tn7 sites of E. coli strains were generated using the pUC18mini R6K vector with the target genes amplified by PCR and inserted into the vector through Gibson assembly at the XhoI and SacI restriction sites [100]. .. E. coli S17-1 λ pir donor strains carrying the mutant constructs and E. coli ATCC-11775 recipient strains to be mutated were grown overnight on LB plates containing appropriate antibiotics, then scraped together to create a 1:1 mixture of each donor-recipient pair that was spread on an LB agar plate and incubated at 37 °C for 6 h to facilitate plasmid transfer via conjugation. .. Cell mixtures were then scraped into LB and plated on LB agar containing kanamycin and chloramphenicol to select for E. coli ATCC-11775 with integrated plasmid.

    Article Title: An Interbacterial Cysteine Protease Toxin Inhibits Cell Growth by Targeting Type II DNA Topoisomerases GyrB and ParE
    Article Snippet: To generate mutations of E. coli ATCC-11775 strains, mutant constructs in pRE118 plasmid were transformed into E. coli S17-1 λ pir. .. E. coli S17-1 λ pir donor strains carrying the mutant constructs and E. coli ATCC-11775 recipient strains to be mutated were grown overnight on LB plates containing appropriate antibiotics, then scraped together to create a 1:1 mixture of each donor-recipient pair that was spread on an LB agar plate and incubated at 37°C for 6 hours to facilitate plasmid transfer via conjugation. .. Cell mixtures were then scraped into LB and plated on LB agar containing kanamycin and chloramphenicol to select for E. coli ATCC-11775 with integrated plasmid.

    Conjugation Assay:

    Article Title: An interbacterial cysteine protease toxin inhibits cell growth by targeting type II DNA topoisomerases GyrB and ParE
    Article Snippet: To generate mutations of E. coli ATCC-11775 strains, mutant constructs in pRE118 plasmid were transformed into E. coli S17-1 λ pir. .. E. coli S17-1 λ pir donor strains carrying the mutant constructs and E. coli ATCC-11775 recipient strains to be mutated were grown overnight on LB plates containing appropriate antibiotics, then scraped together to create a 1:1 mixture of each donor-recipient pair that was spread on an LB agar plate and incubated at 37 °C for 6 h to facilitate plasmid transfer via conjugation. .. Cell mixtures were then scraped into LB and plated on LB agar containing kanamycin and chloramphenicol to select for E. coli ATCC-11775 with integrated plasmid.

    Article Title: An interbacterial cysteine protease toxin inhibits cell growth by targeting type II DNA topoisomerases GyrB and ParE.
    Article Snippet: Insertions at the Tn7 sites of E. coli strains were generated using the pUC18mini R6K vector with the target genes amplified by PCR and inserted into the vector through Gibson assembly at the XhoI and SacI restriction sites [100]. .. E. coli S17-1 λ pir donor strains carrying the mutant constructs and E. coli ATCC-11775 recipient strains to be mutated were grown overnight on LB plates containing appropriate antibiotics, then scraped together to create a 1:1 mixture of each donor-recipient pair that was spread on an LB agar plate and incubated at 37 °C for 6 h to facilitate plasmid transfer via conjugation. .. Cell mixtures were then scraped into LB and plated on LB agar containing kanamycin and chloramphenicol to select for E. coli ATCC-11775 with integrated plasmid.

    Article Title: An Interbacterial Cysteine Protease Toxin Inhibits Cell Growth by Targeting Type II DNA Topoisomerases GyrB and ParE
    Article Snippet: To generate mutations of E. coli ATCC-11775 strains, mutant constructs in pRE118 plasmid were transformed into E. coli S17-1 λ pir. .. E. coli S17-1 λ pir donor strains carrying the mutant constructs and E. coli ATCC-11775 recipient strains to be mutated were grown overnight on LB plates containing appropriate antibiotics, then scraped together to create a 1:1 mixture of each donor-recipient pair that was spread on an LB agar plate and incubated at 37°C for 6 hours to facilitate plasmid transfer via conjugation. .. Cell mixtures were then scraped into LB and plated on LB agar containing kanamycin and chloramphenicol to select for E. coli ATCC-11775 with integrated plasmid.



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    e coli atcc 11775 recipient strains  (ATCC)
    99
    ATCC e coli atcc 11775 recipient strains
    E Coli Atcc 11775 Recipient Strains, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e+coli+atcc+11775+recipient+strains/Escherichia+coli+(Migula)+Castellani+and+Chalmers/pm40424468-332-12-14
    Average 99 stars, based on 1 article reviews
    e coli atcc 11775 recipient strains - by Bioz Stars, 2026-10
    99/100 stars
    		  Buy from Supplier

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